编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Thermotoga Medium

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

Thermotoga Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1017.0mL:

NaCl ............................................................................................ 20.0g

Starch, soluble............................................................................... 5.0g

NiCl2·6H2O ................................................................................... 2.0g

KH2PO4......................................................................................... 0.5g

Na2S·9H2O.................................................................................... 0.5g

Yeast extract.................................................................................. 0.5g

Resazurin ...................................................................................1.0mg

Artificial seawater..................................................................250.0mL

Trace elements solution ...........................................................15.0mL

pH 6.5 ± 0.2 at 25°C

Artificial Seawater:

Composition per liter:

NaCl ............................................................................................ 27.7g

MgSO4·7H2O ................................................................................ 7.0g

MgCl2·6H2O.................................................................................. 5.5g

CaCl2·2H2O................................................................................. 2.25g

KCl.............................................................................................. 0.65g

NaBr.............................................................................................. 0.1g

H3BO3 ......................................................................................30.0mg

SrCl2·6H2O...............................................................................15.0mg

Citric acid.................................................................................10.0mg

KI .............................................................................................0.05mg

Preparation of Artificial Seawater: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O ............................................................................... 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl .............................................................................................. 1.0g

MnSO4·2H2O ............................................................................... 0.5g

CoSO4·7H2O ............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O................................................................................... 0.1g

FeSO4·7H2O.................................................................................. 0.1g

KAI(SO4)2·12H2O....................................................................... 0.02g

CuSO4·5H2O ............................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O ......................................................................... 0.01g

NiCl2·6H2O ............................................................................... 0.025g

Na2SeO3·5H2O...........................................................................0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with

KOH. Add remaining components. Add distilled/deionized water to

1.0L.

Preparation of Medium: Prepare and dispense medium under

100% N2. Add components to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Sparge with 100% N2. Distribute into

tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thermotoga maritima and

Thermotoga neapolitana.

在线客服

Thermotoga Medium