培养基配方

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Thermus brockii Medium

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................ 1.0g

Yeast extract.................................................................................. 1.0g

Salts solution..........................................................................100.0mL

pH 7.6 ± 0.2 at 25°C

Salts Solution:

Composition per liter:

NaNO3 ........................................................................................ 6.89g

KNO3 .......................................................................................... 1.03g

MgSO4·7H2O................................................................................ 1.0g

Nitrilotriacetic acid ....................................................................... 1.0g

CaSO4·2H2O ................................................................................. 0.6g

NaCl......................................................................................... 80.0mg

FeCl3 solution...........................................................................10.0mL

Trace elements solution ...........................................................10.0mL

Preparation of Salts Solution: Add nitrilotriacetic acid to

500.0mL of distilled/deionized water. Adjust pH to 8.2 with 1M NaOH.

Add remaining components. Add distilled/deionized water to 1.0L.

FeCl3 Solution:

Composition per 100.0mL:

FeCl3 ........................................................................................ 28.0mg

Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly.

Trace Elements Solution:

Composition per liter:

MnSO4·H2O .................................................................................. 2.2g

H3BO3 ........................................................................................... 0.5g

ZnSO4·7H2O ................................................................................. 0.5g

CoCl2·6H2O .............................................................................46.0mg

Na2MoO4·2H2O .......................................................................25.0mg

CuSO4 ......................................................................................16.0mg

H2SO4.........................................................................................0.5mL

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thermus brockii.