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Thermus Enhanced Agar

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 28.0g

Pancreatic digest of casein............................................................ 2.5g

Yeast extract.................................................................................. 2.5g

MgCl2·6H2O.................................................................................. 0.2g

Nitrilotriacetic acid ....................................................................... 0.1g

CaSO4·2H2O ............................................................................ 40.0mg

Phosphate buffer solution ......................................................100.0mL

Ferric citrate solution.................................................................0.5mL

Trace elements solution .............................................................0.5mL

Phosphate Buffer Solution:

Composition per liter:

Na2HPO4·12H2O......................................................................... 43.0g

KH2PO4....................................................................................... 5.44g

Preparation of Phosphate Buffer Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C.

Ferric Citrate Solution:

Composition per 10.0mL:

Ferric citrate............................................................................. 24.5mg

Preparation of Ferric Citrate Solution: Add ferric citrate to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Trace Elements Solution:

Composition per liter:

Nitrilotriacetic acid ..................................................................... 12.8g

FeCl2·4H2O................................................................................... 1.0g

MnCl2·4H2O ................................................................................. 0.5g

CoCl2·6H2O .................................................................................. 0.3g

CuCl2·2H2O ............................................................................. 50.0mg

Na2MoO4·2H2O ....................................................................... 50.0mg

H3BO3 ...................................................................................... 20.0mg

NiCl2·6H2O.............................................................................. 20.0mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with

KOH. Add remaining components. Add distilled/deionized water to

1.0L.

Preparation of Medium: Add components, except phosphate buf

fer solution, to distilled/deionized water and bring volume to 900.0mL.

Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2 with

NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–

55°C. Aseptically add 100.0mL of sterile phosphate buffer solution.

Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation of Thermus species.

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Thermus Enhanced Agar