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Thermus Medium (DSMZ Medium 1033)

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

Thermus Medium

(DSMZ Medium 1033)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptone........................................................................................ 1.0g

Yeast extract.................................................................................. 1.0g

Mineral solution 1 .................................................................100.0mL

Mineral solution 2 ...................................................................10.0mL

Ferric chloride solution............................................................10.0mL

pH 8.2 ± 0.2 at 25°C

Mineral Solution 1:

Composition per liter:

NaNO3 .......................................................................................... 6.9g

KNO3 ............................................................................................ 1.8g

Na2HPO4..................................................................................... 1.11g

MgSO4·7H2O................................................................................ 1.0g

Nitrilotriacetic acid ....................................................................... 1.0g

CaSO4·2H2O ................................................................................. 0.6g

NaCl............................................................................................ 0.08g

Preparation of Mineral Solution 1: Add nitrilotriacetic acid to

500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5

with KOH. Add remaining components. Add distilled/deionized water

to and bring volume to 1.0L. Mix thoroughly.

Mineral Solution 2:

Composition per liter:

MnSO4·2H2O.............................................................................. 0.22g

ZnSO4·7H2O ............................................................................... 0.05g

H3BO3 ......................................................................................... 0.05g

CuSO4·5H2O.............................................................................. 2.5mg

Preparation of Mineral Solution 2: Add components to distilled/

deionized water to 1.0L. Mix thoroughly.

Ferric Chloride Solution:

Composition per 100.0mL:

FeCl3·6H2O................................................................................ 4.6mg

Preparation of Ferric Chloride Solution: Add FeCl3·6H2O to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.2 with

1M NaOH. Dispense into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C.

Use: For the cultivation of Thermus spp.