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Thermus 162 Medium (DSMZ Medium 878)

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

Thermus 162 Medium

(DSMZ Medium 878)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 28.0g

Yeast extract.................................................................................. 1.0g

Tryptone........................................................................................ 1.0g

MgCl2·6H2O .......................................................................... 200.0mg

Nitrilotriacetic acid ................................................................ 100.0mg

CaSO4·2H2O ............................................................................ 40.0mg

Phosphate buffer ....................................................................100.0mL

Ferric citrate solution.................................................................0.5mL

Trace elements solution .............................................................0.5mL

pH 7.2 ± 0.2 at 25°C

Ferric Citrate Solution:

Composition per 10.0mL:

Ferric citrate............................................................................. 24.5mg

Preparation of Ferric Citrate Solution: Add ferric citrate to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Trace Elements Solution:

Composition per liter:

CoCl2·6H2O ................................................................................ 45.0g

CuSO4·5H2O............................................................................... 25.0g

Na2MoO4·4H2O .......................................................................... 25.0g

MnSO4·2H2O.............................................................................. 2.28g

ZnSO4·7H2O ................................................................................. 0.5g

H3BO3 ........................................................................................... 0.5g

H2SO4.........................................................................................0.5mL

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Phosphate Buffer:

Composition per liter:

Na2HPO4·12H2O......................................................................... 43.0g

KH2PO4....................................................................................... 5.44g

Preparation of Phosphate Buffer: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH

to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Add components, except phosphate buf

fer, to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°C. Add 100.0mL warm phosphate

buffer. Mix thoroughly. Pour into Petri dishes or distribute into sterile

tubes.

Use: For the cultivation and maintenance of Thermus spp., Rubrobacter

xylanophilus, Thermonema rossianum, Deinococcus geothermalis,

Deinococcus murrayi, and Tepidimonas ignava.

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Thermus 162 Medium (DSMZ Medium 878)