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Thiobacillus acidophilus Agar

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

(NH4)2SO4..................................................................................... 3.0g

MgSO4·7H2O ............................................................................... 1.0g

KH2PO4......................................................................................... 0.5g

KCl................................................................................................ 0.1g

Ca(NO3)2·4H2O ....................................................................... 18.0mg

FeSO4·7H2O............................................................................. 0.01mg

Glucose solution ......................................................................20.0mL

pH 4.5 ± 0.2 at 25°C

Glucose Solution:

Composition per 20.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except glucose solu

tion, to distilled/deionized water and bring volume to 980.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C. Adjust pH to 4.5 with

H2SO4. Aseptically add 20.0mL of sterile glucose solution. Mix thor

oughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Thiobacillus acidophilus.