培养基配方

您现在的位置：网站首页 >> 培养基配方

Thiobacillus acidophilus Medium (DSMZ Medium 108)

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus acidophilus Medium

(DSMZ Medium 108)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

(NH4)2SO4 .................................................................................... 3.0g

MgSO4·7H2O................................................................................ 1.0g

KH2PO4......................................................................................... 0.5g

KCl............................................................................................... 0.1g

Ca(NO3)2·4H2O ....................................................................... 18.0mg

FeSO4·7H2O............................................................................. 0.01mg

Glucose solution ......................................................................50.0mL

pH 4.5 ± 0.2 at 25°C

Glucose Solution:

Composition per 100.0mL:

D-Glucose.................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except glucose solu

tion, to distilled/deionized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL

sterile glucose solution. Mix thoroughly. Adjust pH to 4.5 with H2SO4.

Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Acidiphilium acidophi

lum.