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Thiobacillus aquaesulis Medium

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution B ..............................................................................900.0mL

Solution A ..............................................................................100.0mL

Solution A:

Composition per 100.0mL:

Na2HPO4·2H2O............................................................................. 7.9g

KH2HPO4 ...................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.6.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution B:

Composition per 900.0mL:

Agar ............................................................................................ 15.0g

Na2S2O3 ........................................................................................ 5.0g

NH4Cl ........................................................................................... 0.4g

MgSO4·7H2O ................................................................................ 0.1g

Phenol Red.................................................................................3.0mg

Trace metals .............................................................................10.0mL

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C.

Trace Metals:

Composition per liter:

EDTA .......................................................................................... 50.0g

NaOH .......................................................................................... 11.0g

ZnSO4·7H2O ............................................................................... 11.0g

CaCl2·2H2O................................................................................. 7.34g

FeSO4·7H2O.................................................................................. 5.0g

MnCl2·4H2O.................................................................................. 2.5g

CoCl2·6H2O .................................................................................. 0.5g

(NH4)2Mo2O27 .............................................................................. 0.5g

CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Metals: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0

with H2SO4.

Preparation of Medium: Aseptically combine 100.0mL of sterile

solution A with 900.0mL of sterile solution B. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation of Thiobacillus aquaesulis.