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Thiobacillus intermedius Medium

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Glucose ....................................................................................... 10.0g

Na2S2O3·5H2O ............................................................................ 10.0g

Solution I ......................................................................................1.0L

Solution II ................................................................................10.0mL

Solution I:

Composition per liter:

NH4Cl ........................................................................................... 1.0g

K2HPO4......................................................................................... 0.6g

MgCl2·6H2O ................................................................................. 0.5g

KH2PO4......................................................................................... 0.4g

MgSO4 .......................................................................................... 0.3g

CaCl2·2H2O .................................................................................. 0.2g

FeCl3·6H2O................................................................................. 0.02g

Preparation of Solution I: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly.

Solution II:

Composition per liter:

CaCl2·2H2O .................................................................................. 0.1g

ZnSO4·7H2O ............................................................................... 0.09g

CuSO4·5H2O............................................................................... 0.04g

MnSO4 ........................................................................................ 0.02g

Na2B4O7...................................................................................... 0.01g

(NH4)6Mo7O24·4H2O ................................................................. 5.0mg

Preparation of Solution II: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine 1.0L of solution I, 10.0mL of

solution II, 10.0g of glucose, and 10.0g of Na2S2O3·5H2O. Mix thor

oughly. Filter sterilize. Aseptically distribute into sterile tubes or

flasks.

Use: For the isolation and mixotrophic cultivation of Thiobacillus

intermedius.