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Thiobacillus thioparusii Agar

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1003.0mL:

Solution B ..............................................................................900.0mL

Solution A ..............................................................................100.0mL

Vitamin solution.........................................................................3.0mL

pH 7.1 ± 0.2 at 25°C

Solution A:

Composition per 100.0mL:

K2HPO4......................................................................................... 2.0g

KH2PO4......................................................................................... 2.0g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution B:

Composition per 900.0mL:

Agar ............................................................................................ 15.0g

Na2S2O3·5H2O .............................................................................. 5.0g

Na2CO3 ......................................................................................... 0.4g

NH4Cl ........................................................................................... 0.4g

MgCl2·6H2O.................................................................................. 0.2g

Bromcresol Purple (saturated aqueous solution) .......................2.0mL

Trace elements solution .............................................................1.0mL

Trace Elements Solution:

Composition per liter:

Disodium EDTA ......................................................................... 50.0g

NaOH .......................................................................................... 11.0g

ZnSO4·7H2O ............................................................................... 11.0g

CaCl2·2H2O................................................................................. 7.34g

FeSO4·7H2O.................................................................................. 5.0g

MnCl2·4H2O.................................................................................. 2.5g

CoCl2·6H2O .................................................................................. 0.5g

(NH4)6MoO24·4H2O...................................................................... 0.5g

CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 6.0.

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C.

Vitamin Solution:

Composition per liter:

Calcium DL-pantothenate......................................................... 20.0mg

Nicotinic acid........................................................................... 20.0mg

Pyridoxine·HCl ........................................................................ 20.0mg

Riboflavin ................................................................................ 20.0mg

p-Aminobenzoic acid............................................................... 10.0mg

Thiamine·HCl .......................................................................... 10.0mg

Biotin ......................................................................................... 1.0mg

Vitamin B12 ................................................................................ 1.0mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH

to 7.0. Filter sterilize.

Preparation of Medium: Aseptically combine 100.0mL of sterile

solution A with 900.0mL of sterile solution B and 3.0mL of sterile vi

tamin solution. Mix thoroughly. Pour into sterile Petri dishes or distrib

ute into sterile tubes.

Use: For the cultivation and maintenance of Thiobacillus thioparus.

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Thiobacillus thioparusii Agar