培养基配方

您现在的位置：网站首页 >> 培养基配方

Tieghemiomyces Medium

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Casein hydrolysate...................................................................... 10.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.5g

ZnSO4·7H2O ..............................................................................0.2mg

FeSO4 .........................................................................................0.2mg

MnSO4·H2O ...............................................................................0.2mg

Thiamine ....................................................................................0.2mg

Biotin .......................................................................................0.01mg

Glycerol ...................................................................................20.0mL

pH 6.0–6.5 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Adjust pH to 6.0–6.5. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the cultivation and maintenance of Tieghemiomyces parasit

icus.