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Tindallia Medium (DSMZ Medium 798)

[所属分类:培养基配方] [发布时间:2021-9-16] [发布人:网站管理员2] [阅读次数:] [返回]

Tindallia Medium

(DSMZ Medium 798)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 10.0g

Na2CO3 ......................................................................................... 8.0g

Yeast extract.................................................................................. 4.0g

NH4Cl ........................................................................................... 0.5g

KCl................................................................................................ 0.2g

K2HPO4......................................................................................... 0.2g

Na2S·9H2O solution.................................................................10.0mL

Trace elements solution SL-10 ..................................................1.0mL

Vitamin solution.........................................................................1.0mL

pH 9.0 ± 0.2 at 25°C

Vitamin Solution:

Composition per liter:

Pyridoxine-HCl........................................................................ 10.0mg

Thiamine-HCl·2H2O.................................................................. 5.0mg

Riboflavin .................................................................................. 5.0mg

Nicotinic acid............................................................................. 5.0mg

D-Ca-pantothenate ..................................................................... 5.0mg

p-Aminobenzoic acid................................................................. 5.0mg

Lipoic acid ................................................................................. 5.0mg

Biotin ......................................................................................... 2.0mg

Folic acid ................................................................................... 2.0mg

Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Sparge

with 80% H2 + 20% CO2. Filter sterilize.

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Sparge with N2.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store an

aerobically.

Preparation of Medium: Prepare and dispense medium under

100% N2. Add components, except vitamin solution, to distilled/deion

ized water and bring volume to 999.0mL. Mix thoroughly. Gently heat

and bring to boiling. Boil for 5 min. Cool to 25°C while sparging with

100% N2. Adjust pH to 9.0. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C. Aseptically and anaerobically add 1.0mL vita

min solution. Mix thoroughly. Aseptically and anaerobically under

100% N2 distribute into sterile tubes or bottles.

Use: For the cultivation of Tindallia magadiensis.

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Tindallia Medium (DSMZ Medium 798)