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Tinsdale Agar

[所属分类:培养基配方] [发布时间:2021-9-16] [发布人:网站管理员2] [阅读次数:] [返回]

Tinsdale Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Proteose peptone......................................................................... 20.0g

Agar ............................................................................................ 15.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 5.0g

L-Cystine ..................................................................................... 0.24g

Tinsdale supplement ..............................................................150.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems and Oxoid Unipath.

Tinsdale Supplement:

Composition per 100.0mL:

Na2S2O3 ...................................................................................... 0.43g

K2TeO3........................................................................................ 0.35g

Serum.....................................................................................100.0mL

Caution: Potassium tellurite is toxic.

Preparation of Tinsdale Supplement: Add Na2S2O3 and K2TeO3

to serum. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except Tinsdale sup

plement, to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of

sterile Tinsdale supplement. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the primary isolation and identification of Corynebacterium

diphtheriae.

Tinsdale HiVeg Agar Base with Tinsdale Supplement

Composition per liter:

Plant peptone .............................................................................. 20.0g

Agar ............................................................................................ 15.0g

NaCl.............................................................................................. 5.0g

Na2S2O3 ...................................................................................... 0.43g

L-Cystine..................................................................................... 0.24g

Tinsdale supplement ..............................................................150.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Tinsdale Supplement:

Composition per 100.0mL:

Na2S2O3 ...................................................................................... 0.43g

K2TeO3........................................................................................ 0.35g

Serum.....................................................................................100.0mL

Caution: Potassium tellurite is toxic.

Preparation of Tinsdale Supplement: Add Na2S2O3 and K2TeO3

to serum. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except Tinsdale sup

plement, to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of

sterile Tinsdale supplement. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the primary isolation and identification of Corynebacterium

diphtheriae.

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Tinsdale Agar