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Todd-Hewitt Broth

[所属分类:培养基配方] [发布时间:2021-9-17] [发布人:网站管理员2] [阅读次数:] [返回]

Todd-Hewitt Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from.......................................................... 500.0g

Neopeptone................................................................................. 20.0g

Na2CO3 ......................................................................................... 2.5g

Glucose ......................................................................................... 2.0g

NaCl.............................................................................................. 2.0g

Na2HPO4....................................................................................... 0.4g

pH 7.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of group A streptococci used in serological

typing, and for the cultivation of a variety of pathogenic microorgan

isms.