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Treponema Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Treponema Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Spirolate agar.........................................................................675.0mL

Brain heart infusion broth......................................................225.0mL

Rabbit serum,

inactivated at 56°C for 30 min ........................................100.0mL

pH 7.0–7.2 ± 0.2 at 25°C

Spirolate Agar:

Composition per 675.0mL:

Pancreatic digest of casein.......................................................... 15.0g

Ionagar No. 2 ................................................................................ 8.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl.............................................................................................. 2.5g

L-Cysteine·HCl·H2O...................................................................... 1.0g

Sodium thioglycolate.................................................................... 0.5g

Palmitic acid ............................................................................... 0.05g

Stearic acid.................................................................................. 0.05g

Oleic acid.................................................................................... 0.05g

Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Agar: Add components to distilled/de

ionized water and bring volume to 675.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Brain Heart Infusion Broth:

Composition per liter:

Pancreatic digest of gelatin......................................................... 14.5g

Brain heart, solids from infusion .................................................. 6.0g

Peptic digest of animal tissue........................................................ 6.0g

NaCl .............................................................................................. 5.0g

Casein............................................................................................ 5.0g

Glucose ......................................................................................... 3.0g

Na2HPO4 ....................................................................................... 2.5g

Preparation of Brain Heart Infusion Broth: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Aseptically combine 675.0mL of cooled,

sterile spirolate agar, 225.0mL of cooled, sterile brain heart infusion

broth, and 100.0mL of rabbit serum. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the isolation of oral treponemes.