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Treponema Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Treponema Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Cysteine·HCl·H2O ........................................................................ 1.0g

Glucose ......................................................................................... 1.0g

Nicotinamide................................................................................. 0.4g

Spermidine·4HCl ........................................................................ 0.15g

Sodium isobutyrate ..................................................................... 0.02g

Thiamine pyrophosphate ........................................................... 5.0mg

PPLO broth ............................................................................900.0mL

Rabbit serum, inactivated ......................................................100.0mL

pH 7.8 ± 0.2 at 25°C

PPLO Broth:

Composition per 900.0mL:

Beef heart, infusion from solids.................................................. 50.0g

Peptone ....................................................................................... 10.0g

NaCl.............................................................................................. 5.0g

Preparation of PPLO Broth: Add components to distilled/deion

ized water and bring volume to 900.0mL. Mix thoroughly.

Preparation of Medium: Combine components, except rabbit se

rum. Mix thoroughly. Filter sterilize. Aseptically add sterile rabbit se

rum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of oral treponemes. For the cultivation of

Treponema denticola, Treponema macrodentium, and Treponema ora

lis.