培养基配方

您现在的位置：网站首页 >> 培养基配方

Trichomonas HiVeg Agar Base with Serum and Selective Supplement

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trichomonas HiVeg Agar Base

with Serum and Selective Supplement

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant extract No. 2 ...................................................................... 25.0g

NaCl.............................................................................................. 6.5g

Glucose ......................................................................................... 5.0g

Agar .............................................................................................. 1.0g

Horse serum.............................................................................80.0mL

Selective supplement ...............................................................10.0mL

pH 6.4 ± 0.2 at 25°C

Source: This medium, without horse serum or selective supplement,

is available as a premixed powder from HiMedia.

Horse Serum:

Composition per 80.0mL:

Horse serum.............................................................................80.0mL

Preparation of Horse Serum: Gently heat sterile horse serum to

56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use

immediately.

Selective Supplement:

Composition per 10.0mL:

Streptomycin................................................................................. 0.5g

Penicllin ............................................................................ 1,000,000U

Preparation of Selective Supplement: Add components to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except horse serum

and selective supplement, to distilled/deionized water and bring vol

ume to 910.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 80.0mL of freshly prepared sterile horse serum and

10.0mL sterile selective supplement. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the cultivation of Trichomonas vaginalis.