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Tryptic Nitrate Medium

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ...................................................................................... 20.0g

Na2HPO4 ....................................................................................... 2.0g

Agar .............................................................................................. 1.0g

Glucose ......................................................................................... 1.0g

KNO3 ............................................................................................ 1.0g

pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15

min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of Pseudomonas and

related genera. For the differentiation of bacteria based on their reduc

tion of nitrate to nitrite. After incubation of the bacterium in tryptic

nitrate medium for 18–24 hr, sulfanillic acid and α-naphthol reagents

are added. Nitrate reduction is indicated by the development of a red to

violet color.