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Tryptic Soy Agar with Magnesium

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Sulfate and Sodium Chloride

Composition per liter:

Pancreatic digest of casein.......................................................... 50.0g

NaCl............................................................................................ 30.0g

Agar ............................................................................................ 15.0g

Pancreatic digest of soybean meal................................................ 5.0g

MgSO4·7H2O................................................................................ 1.5g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes in 20.0mL volumes.

Use: For the cultivation of Vibrio species from foods.