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Tryptic Soy Agar with 0.6% Yeast Extract

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]
Tryptic Soy Agar with 0.6% Yeast Extract

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g
Pancreatic digest of casein.......................................................... 15.0g
Yeast extract.................................................................................. 6.0g
Pancreatic digest of soybean meal................................................ 5.0g
NaCl.............................................................................................. 5.0g
pH 7.0–7.5 at 25°C
Source: This medium is available as a premixed powder from BD Di
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of Listeria monocytogenes from
foods.
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