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Tryptic Soy Broth with 0.001M Calcium Chloride (ATCC Medium 1380)

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptic Soy Broth with 0.001M Calcium Chloride

(ATCC Medium 1380)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 18.0g

Papaic digest of soybean meal ...................................................... 6.0g

NaCl .............................................................................................. 6.0g

CaCl2 solution ..........................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium, without CaCl2, is available as a premixed pow

der from BD Diagnostic Systems.

Calcium Chloride Solution:

Composition per 10.0mL:

CaCl2......................................................................................... 0.111g

Preparation of Calcium Chloride Solution: Add CaCl2 to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except CaCl2 solution,

to distilled/deionized water and bring volume to 990mL. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Aseptically add 10.0mL CaCl2 solution. Mix thor

oughly. Distribute into tubes or flasks.

Use: For the isolation and cultivation of Bronchothrix thermospacta.