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Tryptic Soy Fast Green Agar (TSFA)

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptic Soy Fast Green Agar

(TSFA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

Agar ............................................................................................ 15.0g

NaCl.............................................................................................. 5.0g

Papaic digest of soybean meal...................................................... 3.0g

K2HPO4......................................................................................... 2.5g

Glucose ......................................................................................... 2.5g

Fast Green FCF........................................................................... 0.25g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically adjust pH to 7.3.

Pour into sterile Petri dishes.

Use: For the isolation and cultivation of Salmonella species from

foods.