培养基配方

您现在的位置：网站首页 >> 培养基配方

Trypticase™ Novobiocin Broth (TN Broth)

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Novobiocin Broth

(TN Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

NaCl.............................................................................................. 5.0g

Papaic digest of soybean meal...................................................... 3.0g

K2HPO4......................................................................................... 2.5g

Glucose ......................................................................................... 2.5g

Bile salts No. 3.............................................................................. 1.5g

K2HPO4......................................................................................... 1.5g

Novobiocin solution.................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Novobiocin Solution:

Composition per 10.0mL:

Novobiocin ................................................................................. 0.02g

Preparation of Novobiocin Solution: Add novobiocin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except novobiocin solu

tion, to distilled/deionized water and bring volume to 990.0mL. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add sterile novobiocin

solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation of verotoxin-producing Escherichia coli.