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Trypticase™ Serum Seawater Agar

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 12.0g

Pancreatic digest of casein............................................................ 1.0g

Seawater.................................................................................990.0mL

Horse serum .............................................................................10.0mL

Preparation of Medium: Add agar and pancreatic digest of casein

to 990.0mL of seawater. Mix thoroughly. Gently heat and bring to boil

ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 10.0mL of sterile horse serum. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Diplophyrs marina, Hali

phthoros milfordensis, and Ostracoblabe implexa.