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Trypticase™ Soy Agar with Cefazidime (ATCC Medium 1997)

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar with Cefazidime

(ATCC Medium 1997)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Agar ............................................................................................ 15.0g

Papaic digest of soybean meal...................................................... 5.0g

NaCl.............................................................................................. 5.0g

Ceftazidime solution................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Ceftazidime Solution:

Composition per 10.0mL:

Ceftazidime................................................................................ 4.0mg

Preparation of Ceftazidime Solution: Add ceftazidime to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except ceftazidime so

lution, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of

sterile ceftazidime solution. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Klebsiella oxytoca.