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Trypticase™ Soy Agar with Lecithin and Polysorbate 80 (Microbial Content Test Agar)

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar

with Lecithin and Polysorbate 80

(Microbial Content Test Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Agar ............................................................................................ 15.0g

Papaic digest of soybean meal ...................................................... 5.0g

NaCl .............................................................................................. 5.0g

Polysorbate 80 (Tween™ 80) ....................................................... 5.0g

Lecithin ......................................................................................... 0.7g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13

psi pressure–118°C. Cool to 45°–50°C. Pour into sterile Petri dishes in

17.0mL volumes or leave in tubes.

Use: For the detection and enumeration of microorganisms in replicate

plating techniques. Also used for the detection and enumeration of

microorganisms present on surfaces of sanitary importance.