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Trypticase™ Soy Agar with Sodium Chloride, Horse Serum, and Penicillin

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Agar with Sodium Chloride,

Horse Serum, and Penicillin

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 35.0g

Pancreatic digest of casein.......................................................... 15.0g

Agar ............................................................................................ 15.0g

Papaic digest of soybean meal ...................................................... 5.0g

Horse serum, inactivated........................................................100.0mL

Penicillin solution ....................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Penicillin Solution:

Composition per 10.0mL:

Penicillin ........................................................................... 1,000,000U

Preparation of Penicillin Solution: Add penicillin to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except horse serum

and penicillin solution, to distilled/deionized water and bring volume

to 890.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Do not overheat. Cool to

50°C. Aseptically add 100.0mL of sterile horse serum and 10.0mL of

sterile penicillin solution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the isolation and cultivation of fungi.