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Trypticase™ Soy Broth (Soybean Casein Digest Broth, USP) (LMG Medium 185)

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Broth

(Soybean Casein Digest Broth, USP)

(LMG Medium 185)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

NaCl .............................................................................................. 5.0g

Papaic digest of soybean meal ...................................................... 3.0g

K2HPO4......................................................................................... 2.5g

Glucose ......................................................................................... 2.5g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bordetella spp., Capno

cytophaga spp., Brachybacterium spp., Erwinia amylovora, Curtobac

terium flaccumfaciens, Aeromonas spp., Ralstonia spp., Ornithobacte

rium rhinotracheale, Riemerella spp., Arthrobacter spp., Pedobacter

spp., Burkholderia vietnamiensis, Myroides spp., Chryseobacterium

spp., Microbacterium spp., Nocardioides spp., Cellulomonas spp.,

Agromyces spp., Pelistega europaea, Bergeyella zoohelcum, Bacillus

spp., Burkholderia spp., Brevibacillus spp., Aneurinibacillus aneurini

lyticus, Pandoraea spp., and Pseudomonas spp. For the cultivation of

a wide variety of fastidious and nonfastidious microorganisms from

clinical and nonclinical specimens. Also used for the rapid estimation

of the bacteriological quality of water.