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Trypticase™ Soy Broth Agar Modified (DSMZ Medium 535a)

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Broth Agar Modified

(DSMZ Medium 535a)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein.......................................................... 17.0g

NaCl.............................................................................................. 5.0g

Pancreatic digest of soybean meal................................................ 3.0g

K2HPO4......................................................................................... 2.5g

Glucose ......................................................................................... 2.5g

Horse blood, defibrinated ........................................................50.0mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse blood, to

distilled/deionized water and bring volume to 950.0mL. Mix thorough

ly. Heat with frequent agitation and boil for 1 min to completely dis

solve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add 50.0mL of sterile, defibrinated horse blood. Mix

thoroughly and pour into sterile Petri dishes.

Use: For cultivation and maintenance of Corynebacterium glucurono

lyticum, Corynebacterium durum, Corynebacterium falsenii, Coryne

bacterium singulare, and Corynebacterium xerosis.