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Trypticase™ Soy Broth, Modified

[所属分类:培养基配方] [发布时间:2021-9-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1000.2mL:

Pancreatic digest of casein.......................................................... 17.0g

NaCl ............................................................................................ 15.0g

K2HPO4 ........................................................................................ 4.0g

Papaic digest of soybean meal ...................................................... 3.0g

Glucose ......................................................................................... 2.5g

Bile salts No. 3.............................................................................. 1.5g

Novobiocin solution...................................................................0.2mL

pH 7.3 ± 0.2 at 25°C

Novobiocin Solution:

Composition per liter:

Novobiocin.................................................................................. 0.05g

Preparation of Novobiocin Solution: Add novobiocin to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except novobiocin so

lution, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add sterile novobiocin

solution. Mix thoroughly. Aseptically distribute into sterile tubes.

Use: For the isolation and cultivation of Shigella species from food.