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Trypticase™ Soy Broth with Sheep Blood (LMG Medium 189)

[所属分类:培养基配方] [发布时间:2021-9-26] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Soy Broth with Sheep Blood

(LMG Medium 189)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

NaCl .............................................................................................. 5.0g

Papaic digest of soybean meal ...................................................... 3.0g

K2HPO4......................................................................................... 2.5g

Glucose ......................................................................................... 2.5g

Sheep blood, defibrinated ........................................................50.0mL

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 950.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix thor

oughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Bartonella bacilliformis.