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Trypticase™ Soy Yeast Extract Agar

[所属分类:培养基配方] [发布时间:2021-9-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 28.0g

NaCl ............................................................................................ 10.0g

Pancreatic digest of casein............................................................ 2.5g

Yeast extract.................................................................................. 2.5g

Base medium..........................................................................100.0mL

Phosphate buffer ....................................................................100.0mL

pH 7.2 ± 0.2 at 25°C

Base Medium:

Composition per liter:

MgCl2·6H2O ................................................................................. 2.0g

Titriplex I ...................................................................................... 1.0g

CaSO4·2H2O................................................................................. 0.4g

NaOH pellets................................................................................. 0.2g

Fe-citrate solution ......................................................................5.0mL

Trace elements solution .............................................................5.0mL

Fe-Citrate Solution:

Composition per 100.0mL:

Fe-citrate..................................................................................... 0.25g

Preparation of Fe-Citrate Solution: Add Fe-citrate to distilled/

deionized water and bring volume to 100.0mL. Mix thoroughly.

Trace Elements Solution:

Composition per 5.0mL:

CuCl2·2H2O............................................................................. 50.0mg

Titriplex I ................................................................................. 12.8mg

FeCl2·4H2O................................................................................ 1.0mg

MnCl2·4H2O .............................................................................. 0.5mg

CoCl2·6H2O............................................................................... 0.3mg

ZnCl2 ......................................................................................... 0.2mg

Na2MoO4·2H2O....................................................................... 50.0mg

NiCl2·6H2O.............................................................................. 50.0mg

H3BO3...................................................................................... 20.0mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 5.0mL. Mix thoroughly.

Preparation of Base Medium: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly.

Phosphate Buffer:

Composition per liter:

Na2HPO4·12H2O ........................................................................ 43.0g

KH2PO4 ...................................................................................... 5.44g

Preparation of Phosphate Buffer: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Add components, except phosphate buf

fer, and bring volume to 900.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C. Aseptically add 100.0mL of sterile phosphate buffer. Mix

thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Lactococcus lactis and

Lactococcus plantarum.

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Trypticase™ Soy Yeast Extract Agar