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Trypticase™ Yeast Extract Glucose Medium

[所属分类:培养基配方] [发布时间:2021-9-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 10.0g

Glucose ....................................................................................... 10.0g

KH2PO4......................................................................................... 6.8g

Yeast extract.................................................................................. 5.0g

NaHCO3........................................................................................ 1.0g

Tween™ 80................................................................................... 0.5g

Sodium formaldehyde sulfoxalate ................................................ 0.5g

CaCl2........................................................................................... 0.02g

MgSO4 ........................................................................................ 0.02g

NaCl............................................................................................ 0.02g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Propionibacterium species.