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Tryptone Glucose Beef Extract Agar (Tryptone Glucose Extract Agar)

[所属分类:培养基配方] [发布时间:2021-9-26] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Glucose Beef Extract Agar

(Tryptone Glucose Extract Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein............................................................ 5.0g

Beef extract................................................................................... 3.0g

Glucose ......................................................................................... 1.0g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. If the dilution of the specimen

is greater than 1:10, add 10.0mL of sterile 10% skim milk solution.

Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the enumeration of bacteria by the standard plate count proce

dure. For the cultivation and enumeration of bacteria from milk and dairy

products. For the detection of thermophilic organisms.