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Tryptone Glucose Medium

[所属分类:培养基配方] [发布时间:2021-9-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Glucose ......................................................................................... 5.0g

K2HPO4....................................................................................... 0.35g

KH2PO4......................................................................................... 0.2g

MgCl2·6H2O.................................................................................. 0.1g

CaCl2·2H2O................................................................................. 0.07g

Vitamin solution.......................................................................10.0mL

Vitamin Solution:

Composition per 10.0mL:

Thiamine .................................................................................200.0μg

Biotin ........................................................................................50.0μg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except vitamin solu

tion, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Aseptically add 10.0mL of sterile vitamin so

lution. Mix thoroughly. Aseptically distribute into sterile flasks or

tubes.

Use: For the cultivation and maintenance of Amoebidium parasiticum,

Capniomyces stellatus, Smittium culicis, Smittium culisetae, Smittium

simulii, and Smittium species.