培养基配方

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Tryptone Lactose Iron HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-9-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate ........................................................................ 20.0g

Lactose........................................................................................ 10.0g

Agar .............................................................................................. 3.5g

Na2SO3 ......................................................................................... 0.4g

FeSO4............................................................................................ 0.2g

Na2S2O3 ...................................................................................... 0.08g

Phenol Red.................................................................................. 0.02g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of fastidious aerobic and

facultative microorganisms such as Escherichia coli and Pseudomonas

species.