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Tryptone Thioglycolate Medium (DSMZ Medium 48)

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Thioglycolate Medium

(DSMZ Medium 48)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract.................................................................................. 6.0g

K2HPO4....................................................................................... 5.45g

Peptone ......................................................................................... 2.0g

Tryptone........................................................................................ 2.0g

KH2PO4......................................................................................... 1.2g

Na-thioglycolate ........................................................................... 0.5g

MgSO4·7H2O............................................................................ 0.025g

CaCl2·2H2O .............................................................................. 0.015g

FeSO4·7H2O................................................................................ 0.01g

CoCl2·6H2O ............................................................................... 2.5mg

Na2MoO4·2H2O ......................................................................... 2.5mg

MnCl2·4H2O .............................................................................. 2.0mg

Glucose solution ......................................................................50.0mL

pH 7.5 ± 0.2 at 25°C

Glucose Solution:

Composition per 50.0mL:

D-Glucose.................................................................................... 20.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril

ize.

Preparation of Medium: Add components, except glucose solution,

to distilled/deionized water and bring volume to 950.0mL. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 25°C. Aseptically add 50.0mL of sterile glu

cose solution. Mix thoroughly. Aseptically distribute into sterile tubes or

flasks.

Use: For the cultivation of Clostridium beijerinckii.