培养基配方

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Tryptophan HiVeg Medium

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate......................................................................... 10.0g

NaCl .............................................................................................. 5.0g

DL-Tryptophan .............................................................................. 1.0g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.4.

Filter sterilize. Aseptically distribute in 1.0mL volumes into sterile

screw-capped tubes.

Use: For the cultivation of Flavobacterium species and a variety of

other bacteria. Also used to differentiate bacteria based on indole pro

duction. Indole is determined by the addition of modified Kovacs

reagent to cultures that have incubated for 18–24 hr. Formation of a red

color in the upper layer indicates indole formation.