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Tryptose Agar with Sheep Blood (ATCC Medium 546)

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptose Agar with Sheep Blood

(ATCC Medium 546)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Tryptose ...................................................................................... 10.0g

NaCl .............................................................................................. 5.0g

Beef extract ................................................................................... 3.0g

Sheep blood, defibrinated .............................................50.0–100.0mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 900–950mL. Mix thor

oughly. Gently heat and bring to boiling. Distribute into tubes. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile sheep blood. Mix thoroughly. Pour into sterile

Petri dishes in 17.0mL volumes or distribute into sterile tubes.

Use: For the cultivation and maintenance of a wide variety of fastidi

ous microorganisms.