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Tryptose Blood Agar

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptose Blood Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Proteose peptone No. 3............................................................... 10.0g

Tryptose ...................................................................................... 10.0g

Beef extract................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 5.0g

Sheep blood ...........................................................................100.0mL

L-Cysteine·HCl solution ............................................................2.5mL

L-Cysteine·HCl Solution:

Composition per 10.0mL:

L-cysteine·HCl ............................................................................. 1.0g

Preparation of L-Cysteine·HCl Solution: Dissolve 1.0g of L

cysteine·HCl in distilled/deionized water and bring volume to 10.0mL.

Mix thoroughly. Filter sterilize. Warm to 50°C.

Preparation of Medium: Add components, except sheep blood and

L-cysteine·HCl solution, to distilled/deionized water and bring volume

to 887.5mL. Mix thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Warm

sheep blood to 50°C. Aseptically add 100.0mL of sterile sheep blood

and 2.5mL of sterile L-cysteine·HCl solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Corynebacterium matru

chotii, Propionibacterium propionicum, and Staphylococcus saccharolyt

icus.