培养基配方

您现在的位置：网站首页 >> 培养基配方

Tryptose Blood Agar Base with Yeast Extract, HiVeg

[所属分类:培养基配方] [发布时间:2021-9-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant hydrolysate No. 1............................................................... 10.0g

NaCl.............................................................................................. 5.0g

Plant extract .................................................................................. 3.0g

Yeast extract.................................................................................. 1.0g

Sheep blood, defibrinated ........................................................70.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium, without blood, is available as a premixed pow

der from HiMedia.

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 930.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep

blood. Mix thoroughly. Pour into sterile Petri dishes in 17.0mL vol

umes or distribute into sterile tubes.

Use: For the cultivation and maintenance of a wide variety of fastidi

ous microorganisms. For the isolation of fastidious organisms and

determining hemolytic reactions.