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Tryptose Cycloserine Dextrose Agar

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Tryptose ...................................................................................... 15.0g

Pancreatic digest of soybean meal ................................................ 5.0g

Yeast extract.................................................................................. 5.0g

Ferric ammonium citrate............................................................... 1.0g

Cycloserine solution ................................................................10.0mL

pH 7.6 ± 0.2 at 25°C

Cycloserine Solution:

Composition per 10.0mL:

D-Cycloserine................................................................................ 0.4g

Preparation of Cycloserine Solution: Add D-cycloserine to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except cycloserine so

lution, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile cy

closerine solution. Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the isolation and cultivation of Clostridium species, espe

cially Clostridium botulinum, from foods.