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Tryptose Phosphate Broth, Modified

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Enzymatic digest of casein ......................................................... 20.0g

NaCl .............................................................................................. 5.0g

Na2HPO4 ....................................................................................... 2.5g

Glucose ......................................................................................... 2.0g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Prior to the

inoculation of anaerobic microorganisms, place tubes of sterile medi

um in a 100°C bath for 15 min and cool undisturbed.

Use: For the cultivation of a variety of fastidious microorganisms,

including pneumococci, streptococci, and meningococci.