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Tryptose Sulfite Cycloserine Agar (TSC Agar)

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptose Sulfite Cycloserine Agar

(TSC Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ...................................................................................... 15.0g

Agar ............................................................................................ 14.0g

Beef extract ................................................................................... 5.0g

Pancreatic digest of soybean meal ................................................ 5.0g

Yeast extract.................................................................................. 5.0g

Ferric ammonium citrate............................................................... 1.0g

Na2S2O5 ........................................................................................ 1.0g

Egg yolk emulsion ...................................................................50.0mL

Cycloserine solution ................................................................10.0mL

pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Egg Yolk Emulsion:

Composition:

Chicken egg yolks............................................................................ 11

Whole chicken egg ............................................................................ 1

Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu

tion of saturated mercuric chloride solution for 1 min. Crack eggs and

separate yolks from whites. Mix egg yolks with 1 chicken egg.

Cycloserine Solution:

Composition per 10.0mL:

D-Cycloserine................................................................................ 0.4g

Preparation of Cycloserine Solution: Add cycloserine to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except cycloserine so

lution and egg yolk emulsion, to distilled/deionized water and bring

volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile cycloserine solution and egg yolk emulsion.

Mix thoroughly. Pour into sterile Petri dishes.

Use: For the presumptive identification and enumeration of Clostrid

ium perfringens.