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TSA 5400 Selective Isolation Medium

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Agar ............................................................................................ 15.0g

Papaic digest of soybean meal...................................................... 5.0g

NaCl.............................................................................................. 5.0g

Bovine blood, citrated............................................................100.0mL

Spectinomycin solution ...........................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Spectinomycin Solution:

Composition per 10.0mL:

Spectinomycin .............................................................................. 0.4g

Preparation of Spectinomycin Solution: Add spectinomycin to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except bovine blood

and spectinomycin solution, to distilled/deionized water and bring vol

ume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile bovine blood and 10.0mL of sterile spectinomy

cin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation of Treponema hyodysenteriae.