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Tween™ 80 Hydrolysis Broth

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 125.0mL:

Neutral Red ................................................................................... 0.1g

Solution 1.................................................................................38.9mL

Solution 2.................................................................................61.1mL

Tween™ 80 solution ................................................................25.0mL

pH 7.0 ± 0.2 at 25°C

Solution 1:

Composition per 400.0mL:

KH2PO4....................................................................................... 22.7g

Preparation of Solution 1: Add KH2PO4 to distilled/deionized wa

ter and bring volume to 400.0mL. Mix thoroughly.

Solution 2:

Composition per 400.0mL:

Na2HPO4 ..................................................................................... 23.8g

Preparation of Solution 2: Add Na2HPO4 to distilled/deionized

water and bring volume to 400.0mL. Mix thoroughly.

Tween™ 80 Solution:

Composition per 50.0mL:

Tween™ 80................................................................................. 10.0g

Preparation of Tween™ 80 Solution: Add Tween™ 80 to dis

tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except Tween™ 80 so

lution, to distilled/deionized water and bring volume to 975.0mL. Mix

thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 25.0mL of sterile Tween™ 80 solution. Mix

thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the differentiation of Mycobacterium species. Strains that

hydrolyze Tween™ 80 within 5 days turn the medium pink to red.