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Tween™ 80 Hydrolysis Medium

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 12.0g

Peptone ....................................................................................... 10.0g

Tween™ 80................................................................................. 10.0g

NaCl.............................................................................................. 5.0g

CaCl2............................................................................................. 0.1g

pH 7.2–7.4 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes.

Use: For the cultivation and differentiation of Pseudomonas species

based on their ability to hydrolyze Tween™ 80. Bacteria that hydro

lyze Tween™ 80 appear as colonies surrounded by an opaque zone.