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TYGM-9 Medium

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TYGM-9 Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl.............................................................................................. 7.5g

K2HPO4......................................................................................... 2.8g

Casein digest................................................................................. 2.0g

Gastric mucin................................................................................ 2.0g

Yeast extract.................................................................................. 1.0g

KH2PO4......................................................................................... 0.4g

Bovine serum, heat inactivated................................................30.0mL

Rice starch solution..................................................................30.0mL

Tween™ solution.......................................................................0.5mL

pH 7.4 ± 0.2 at 25°C

Tween™ Solution:

Composition per 100.0mL:

Tween™ 80................................................................................. 10.0g

Preparation of Tween™ Solution: Add Tween™ 80 to absolute

ethanol and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Rice Starch Solution:

Composition per 100.0mL:

Rice starch .................................................................................... 5.0g

Phosphate-buffered saline solution........................................100.0mL

Preparation of Rice Starch Solution: Heat sterilize rice starch at

150°C for 2 hr. Aseptically add 100.0mL of sterile phosphate-buffered

saline solution. Mix thoroughly. Use immediately.

Phosphate-Buffered Saline Solution:

Composition per liter:

NaCl.............................................................................................. 9.0g

Na2HPO4·7H2O......................................................................... 0.795g

KH2PO4..................................................................................... 0.114g

Preparation of Phosphate-Buffered Saline Solution: Add

components to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C.

Preparation of Medium: Add components, except rice starch solu

tion, Tween™ solution, and bovine serum, to distilled/deionized water

and bring volume to 939.5mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 25°C. Aseptically add 30.0mL of sterile

bovine serum, 30.0mL of sterile rice starch solution, and 0.5mL of sterile

Tween™ solution. Mix thoroughly. Aseptically distribute into sterile,

screw-capped tubes or flasks.

Use: For the cultivation of Dientamoeba fragilis, Ditrichomonas honig

bergii, Entamoeba coli, Entamoeba dispar, Entamoeba gingivalis, Enta

moeba insolita, Entamoeba histolytica, Entamoeba moshkovskii, Entam

oeba polecki, Entamoeba ranarum, Pseudotrichomonas keilini, and

Trepomonas agilis.

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TYGM-9 Medium