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TYGS Medium (Tryptone Yeast Extract Glucose Salt Medium)

[所属分类:培养基配方] [发布时间:2021-9-29] [发布人:网站管理员2] [阅读次数:] [返回]

TYGS Medium

(Tryptone Yeast Extract Glucose Salt Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein.......................................................... 10.0g

NaCl .............................................................................................. 8.0g

Yeast extract.................................................................................. 1.0g

CaCl2·2H2O solution..............................................................100.0mL

Glucose solution ....................................................................100.0mL

CaCl2·2H2O Solution:

Composition per 100.0mL:

CaCl2·2H2O................................................................................... 0.3g

Preparation of CaCl2·2H2O Solution: Add the CaCl2·2H2O to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Filter sterilize.

Glucose Solution:

Composition per 100.0mL:

D-Glucose ...................................................................................... 1.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except CaCl2·2H2O so

lution and glucose solution, to distilled/deionized water and bring vol

ume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add the sterile CaCl2·2H2O solution and sterile glucose so

lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation and maintenance of a variety of bacteria.