培养基配方

您现在的位置：网站首页 >> 培养基配方

TYM Basal Medium, Modified 1

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of peptone ....................................................... 20.0g

Yeast extract................................................................................ 10.0g

Maltose.......................................................................................... 5.0g

L-Cysteine·HCl.............................................................................. 1.0g

K2HPO4......................................................................................... 0.8g

KH2PO4......................................................................................... 0.8g

Agar, noble.................................................................................... 0.5g

L-Ascorbic acid ............................................................................. 0.2g

pH 7.8 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar, to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to

7.8 with NaOH. Add the agar. Mix thoroughly. Gently heat and bring to

boiling. Distribute into screw-capped tubes. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation of Trichomonas species.